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A Bibliometric Analysis of Microalgae Research in the World, Europe, and the European Atlantic Area ArchiMer
Rumin, Judith; Nicolau, Elodie; Gonçalves De Oliveira Junior, Raimundo; Fuentes-grünewald, Claudio; Flynn, Kevin J; Picot, Laurent.
A bibliographic database of scientific papers published by authors affiliated worldwide, especially focused in Europe and in the European Atlantic Area, and containing the keywords “microalga(e)” or “phytoplankton” was built. A corpus of 79,020 publications was obtained and analyzed using the Orbit Intellixir software to highlight the evolution of the research domain. Publication rates from 1960 to 2019, organization of the research, collaboration networks between countries and organizations, emerging and fading research concepts, major studied species, and associated concepts, as well as journals publishing microalgae research were considered. As a result, of the 79,020 papers published worldwide, 26,137 included authors from Europe (33% of world...
Tipo: Text Palavras-chave: Biotechnology; European Atlantic Area; Microalgae; Phytoplankton; Research; Market; Bibliometrics.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00605/71725/70191.pdf
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A method to assess algicidal activity of microalgal extracts coupling microalgae produced in stirred closed photobioreactor operating in continuous with pulse amplitude modulated (PAM) fluorometry ArchiMer
Menguy, Eva; Dumontet, Vincent; Coulombier, Noémie; Meriot, Vincent; Le Dean, Loic; Barthelemy, Vanille; Jauffrais, Thierry.
We describe in the present study a quick and reliable method based on chlorophyll a fluorescence to assess putative algicidal effect of different microalgal extracts. We couple microalgal production under chemostat cultivation mode to continuously produce a given microalgae species (e.g. Dunaliella salina in this study) at a stable physiological state to ease comparison between extracts tested; with a non-destructive method based on chlorophyll a fluorescence. Pulse amplitude modulated (PAM) fluorometry was used to assess over time the effect of different microalgal crude extracts on the efficiency of the photosystem II (PSII) of a tested microalgae (Dunaliella salina). • Microalgal production at stationary phase in stirred closed photobioreactor (PBR)...
Tipo: Text Palavras-chave: Bioassay; Cytoxicity; Microalgae; Quantum efficiency; Fv/Fm; Non photochemical quenching; NPQ; Electron transport rate; Phytochemistry; Anti algal.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00643/75524/76400.pdf
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A Physiologically Structured Equation to Consider Quota Heterogeneity in the Droop Model ArchiMer
Mairet, Francis; Baron, Regis.
The Droop model allows to represent microalgae growth limited by a nutrient, using a cell quota (also referred to as variable-yield) approach. Single-cell measurements have revealed quota heterogeneity in phytoplankton collected from field studies. Such heterogeneity can be due, among other factors, to spatial structure (e.g. in biogeochemical cycles in the ocean, or for photobioreactors connected in series). Nonetheless, quota heterogeneity is generally omitted in modelling studies, using an average quota approach, or included in size-structured or individual-based models. Here, we propose a distributed Droop equation to tackle this problem, considering subpopulation growth -in line with Droop macroscopic view- rather than cell division dynamics. We...
Tipo: Text Palavras-chave: Population Balance Model; Variable-yield model; Cell Quota; Phytoplankton; Microalgae.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00600/71187/69542.pdf
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Algicidal effects of aqueous leaf extracts of neem (Azadirachta indica) on Scenedesmus quadricauda (Turp.) de Brébission Acta Botanica
Chia,Mathias Ahii; Akinsanmi,Jamiu Taiwo; Tanimu,Yahuza; Ladan,Zakari.
The application of synthetic algaecides for the control of algae produces by-products that are sometimes toxic to the environment. There is a need for natural and cheap alternatives to synthetic algaecides. In the present study, we investigated the potential of aqueous crude extract of Azadirachta indica to inhibit the growth of Scenedesmus quadricauda. Phytochemical screening of the extract revealed the presence of groups of bioactive compounds that are capable of inhibiting microalgal growth. Chlorophyll a concentration, dry weight production and cell density of microalga decreased with increasing crude extract concentration. After three days of exposure, the 1000 mg/L extract concentration resulted in complete growth inhibition and cell lysis....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Algaecide; Growth inhibition; Microalgae; Neem; Oxidative stress; Phenotypic plasticity.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062016000100001
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Allocation du carbone et métabolisme azoté chez l'haptophyte Tisochrysis lutea ArchiMer
Garnier, Matthieu.
The aim of this thesis is to improve knowledge on mechanisms involved in the response to nitrogen limitation and in lipid accumulation in the microalgae haptophyte Tisochrysis lutea. The wild type strain and a lipid accumulating mutant strain were grown under different nitrogen limitation and starvation and analyzed by functional genomics. Four genes of high-affinity nitrate/nitrite transporter (Nrt2) were identified and characterized to reveal the mechanisms involved in mineral absorption in this species. Transcriptomes of both strains were sequenced and proteins affected by nitrogen starvation and differentially expressed between the two strains were identified. We so identified the functions regulated by nitrogen deficiency and potentially involved in...
Tipo: Text Palavras-chave: Microalgues; Métabolisme; Azote; Carbone; Lipides; Genomique fonctionelle; Protéomique; Transcriptomique; Microalgae; Metabolism; Nitrogen; Carbon; Lipids; Functional genomics; Proteomics; Transcriptomics.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00353/46378/46000.pdf
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Analysis of Scientific Research Driving Microalgae Market Opportunities in Europe ArchiMer
Rumin, Judith; Nicolau, Elodie; Junior, Raimundo Gonçalves De Oliveira; Fuentes-grünewald, Claudio; Picot, Laurent.
A bibliographic database of scientific papers published by authors affiliated to research institutions worldwide, especially focused in Europe and in the European Atlantic Area, and containing the keywords “microalga(e)” or “phytoplankton” was built. A corpus of 79,020 publications was obtained and analyzed using the Orbit Intellixir software to characterize the research trends related to microalgae markets, markets opportunities and technologies that could have important impacts on markets evolution. Six major markets opportunities, the production of biofuels, bioplastics, biofertilizers, nutraceuticals, pharmaceuticals and cosmetics, and two fast-evolving technological domains driving markets evolution, microalgae harvesting and extraction technologies...
Tipo: Text Palavras-chave: Bibliometric; Microalgae; Biofertilizers; Bioplastics; Biotechnology; Cosmetics; Europe; Food; GMO; Market; Nutraceuticals; Pharmaceuticals; Research.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00630/74185/73788.pdf
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Anti-Biofilm Effect of Biodegradable Coatings Based on Hemibastadin Derivative in Marine Environment ArchiMer
Le Norcy, Tiffany; Niemann, Hendrik; Proksch, Peter; Linossier, Isabelle; Vallee-rehel, Karine; Hellio, Claire; Fay, Fabienne.
Dibromohemibastadin-1 (DBHB) is an already known potent inhibitor of blue mussel phenoloxidase (which is a key enzyme involved in bioadhesion). Within this study, the potentiality of DBHB against microfouling has been investigated. The activity of DBHB was evaluated on key strains of bacteria and microalgae involved in marine biofilm formation and bioassays assessing impact on growth, adhesion and biofilm formation were used. To assess the efficiency of DBHB when included in a matrix, DBHB varnish was prepared and the anti-microfouling activity of coatings was assessed. Both in vitro and in situ immersions were carried out. Confocal Laser Scanning Microscopy (CLSM) was principally used to determine the biovolume and average thickness of biofilms developed...
Tipo: Text Palavras-chave: Antifouling; Biodegradable coating; Hemibastadin; Sponges; Marine bacteria; Microalgae.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00616/72812/72626.pdf
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Antimelanoma activity of Heterocapsa triquetra pigments ArchiMer
Haguet, Quentin; Bonnet, Antoine; Berard, Jean-baptiste; Goldberg, Joelle; Joguet, Nicolas; Fleury, Audrey; Thiery, Valerie; Picot, Laurent.
Heterocapsa triquetra (Ht), a non-toxic dinophyte, was selected to investigate the presence of pigments inhibiting melanoma cells growth. We developed an innovative microwave-assisted extraction process (MAE), determined the Ht pigment composition using Reverse Phase-High Performance Liquid Chromatography (HPLC) and high-resolution Ultra Performance Liquid Chromatography-Mass Spectrometry E (UPLC-MSE) analysis and assessed their antiproliferative activity in human invasive melanoma cells (A2058) grown in vitro. MAE allowed a fast and efficient extraction of Ht pigments as compared with conventional processes (soaking, sonication). Seventeen pigments and derivatives were unequivocally identified by UPLC-MSᴱ in the Ht extract, among which two were supposed...
Tipo: Text Palavras-chave: Dinophyte; Heterocapsa; Melanoma; Microalgae; Microwave assisted extraction; Pigment.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00390/50127/50806.pdf
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Antimicrobial Compounds from Eukaryotic Microalgae against Human Pathogens and Diseases in Aquaculture ArchiMer
Falaise, Charlotte; Francois, Cyrille; Travers, Marie-agnes; Morga, Benjamin; Haure, Joel; Tremblay, Rejean; Turcotte, Francois; Pasetto, Pamela; Gastineau, Romain; Hardivillier, Yann; Leignel, Vincent; Mouget, Jean-luc.
The search for novel compounds of marine origin has increased in the last decades for their application in various areas such as pharmaceutical, human or animal nutrition, cosmetics or bioenergy. In this context of blue technology development, microalgae are of particular interest due to their immense biodiversity and their relatively simple growth needs. In this review, we discuss about the promising use of microalgae and microalgal compounds as sources of natural antibiotics against human pathogens but also about their potential to limit microbial infections in aquaculture. An alternative to conventional antibiotics is needed as the microbial resistance to these drugs is increasing in humans and animals. Furthermore, using natural antibiotics for...
Tipo: Text Palavras-chave: Biological activity; Antimicrobial; Antibacterial; Antifungal; Antiviral; Biological activity; Haslea; Microalgae; Sustainable aquaculture.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00349/46052/45719.pdf
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Antiproliferative Activity of Cyanophora paradoxa Pigments in Melanoma, Breast and Lung Cancer Cells ArchiMer
Baudelet, Paul-hubert; Gagez, Anne-laure; Berard, Jean-baptiste; Juin, Camille; Bridiau, Nicolas; Kaas, Raymond; Thiery, Valerie; Cadoret, Jean-paul; Picot, Laurent.
The glaucophyte Cyanophora paradoxa (Cp) was chemically investigated to identify pigments efficiently inhibiting malignant melanoma, mammary carcinoma and lung adenocarcinoma cells growth. Cp water and ethanol extracts significantly inhibited the growth of the three cancer cell lines in vitro, at 100 mu g.mL(-1). Flash chromatography of the Cp ethanol extract, devoid of c-phycocyanin and allophycocyanin, enabled the collection of eight fractions, four of which strongly inhibited cancer cells growth at 100 mu g.mL(-1). Particularly, two fractions inhibited more than 90% of the melanoma cells growth, one inducing apoptosis in the three cancer cells lines. The detailed analysis of Cp pigment composition resulted in the discrimination of 17 molecules, ten of...
Tipo: Text Palavras-chave: Pigments; Microalgae; Cyanophora paradoxa; Melanoma; Cancer; Apoptosis; Zeaxanthin; Cryptoxanthin.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00177/28818/27289.pdf
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Antiproliferative Activity of Violaxanthin Isolated from Bioguided Fractionation of Dunaliella tertiolecta Extracts ArchiMer
Pasquet, Virginie; Morisset, Perrine; Ihammouine, Said; Chepied, Amandine; Aumailley, Lucie; Berard, Jean-baptiste; Serive, Benoit; Kaas, Raymond; Lanneluc, Isabelle; Thiery, Valerie; Lafferriere, Mathieu; Piot, Jean-marie; Patrice, Thierry; Cadoret, Jean-paul; Picot, Laurent.
Dunaliella tertiolecta (DT) was chemically investigated to isolate molecules inhibiting cancer cell proliferation and inducing apoptosis in vitro. The potency to inhibit cell growth was used for the bio-guided fractionation and isolation of active compounds using chromatographic techniques. The DT dichloromethane extract exhibited a strong anti-proliferative activity on MCF-7 and LNCaP cells, and was further fractionated and sub-fractionated by RP-HPLC. High resolution mass spectrometry and spectrophotometric analysis unequivocally identified violaxanthin as the most antiproliferative molecule present in DT DCM extract. Violaxanthin purified from DT induced MCF-7 dose- dependent growth inhibition in continuous and discontinuous treatments, at...
Tipo: Text Palavras-chave: Pigments; Microalgae; Dunaliella; Violaxanthin; Carotenoid; Cancer; Apoptosis.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00040/15142/12575.pdf
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Bacterial community structure of the marine diatom Haslea ostrearia ArchiMer
Lepinay, Alexandra; Capiaux, Herve; Turpin, Vincent; Mondeguer, Florence; Lebeau, Thierry.
Haslea ostrearia produces a water-soluble, blue-green pigment, called marennine, with proven economic benefits (as a bioactive compound used to green oysters, which improves their market value). Incomplete knowledge of the ecological features of this marine diatom complicates its cultivation. More specifically, the ecology of bacteria surrounding H. ostrearia in ponds is what remains unknown. The structure of this bacterial community was previously analyzed by means of PCR-TTGE before and after isolating H. ostrearia cells recovered from 4 localities in order to distinguish the relative parts of the biotope and biocenose and to describe the temporal dynamics of the bacterial community structure at two time scales (2 weeks vs. 9 months). The bacterial...
Tipo: Text Palavras-chave: Biofilm; Ecology; Metabolic fingerprinting; Microalgae; Phycosphere; TTGE.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00326/43737/43344.pdf
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Bioethanol from Microalgal Biomass: A Promising Approach in Biorefinery BABT
Silva,Carlos Eduardo de Farias; Bertucco,Alberto.
Abstract The development of new technologies which increase the production of biofuel without directly compete with food production is required. Microalgal biomass has recently been in the highlight. The role of this biomass is here discussed within the concept of biorefinery and industrial sustainability of bioethanol production. The process of cultivation in order to accumulate around 50% of carbohydrates in the biomass (dry weight) and the importance of water and nutrient recycling are reviewed. Saccharification of biomass using enzymes or acids and alternative processes such as hydrothermal liquefaction and flash hydrolysis are addressed. Since the main monosaccharide in microalgal biomass is glucose, high rates of hydrolysis and fermentation were,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethanol; Microalgae; Biofuel; Hydrolysis; Nutrient recycle.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100602
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Bioremediation of fishpond effluent and production of microalgae for an oyster farm in an innovative recirculating integrated multi-trophic aquaculture system ArchiMer
Li, Meng; Callier, Myriam; Blancheton, Jean-paul; Galès, Amandine; Nahon, Sarah; Triplet, Sebastien; Geoffroy, Thibault; Menniti, Christophe; Fouilland, Eric; Roque D'Orbcastel, Emmanuelle.
Integrated multi-trophic aquaculture (IMTA) systems are a promising solution for sustainable aquaculture combining nutrient recycling with increased biomass production. An innovative land-based recirculating aquaculture system (RAS) was studied in France for a 60-day experiment. It combined a European sea bass (Dicentrarchus labrax) RAS with two other production systems: high rate algal ponds (HRAP) with natural marine polyspecific algal assemblages, and oysters in separate open tanks. The objective was the assessment of: 1) the efficiency and the stability of the microalgae bioremediation of the effluent from a fish RAS in spring and summer, 2) the abundance and the diversity patterns of the microalgae biomass for consumption in the oyster compartment of...
Tipo: Text Palavras-chave: Microalgae; IMTA; Nutrient bioremediation; Community structure; Oysters.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00480/59207/61900.pdf
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Carbon Dioxide Biofixation and Production of Spirulina sp. LEB 18 Biomass with Different Concentrations of NaNO3 and NaCl BABT
Moraes,Luiza; Rosa,Gabriel Martins da; Souza,Michele da Rosa Andrade Zimmermann de; Costa,Jorge Alberto Vieira.
ABSTRACT Microalgae are efficient at using solar energy to turn CO2 and nutrients into biomass containing lipids, proteins, carbohydrates and other compounds that may be used to produce bioproducts for human and animal consumption and pharmaceutical use. The aim of this study was to assess the effect of the NaNO3 and NaCl concentration on the growth kinetics, the biomass composition and the ability to biofix CO2 using the microalga Spirulina sp. LEB 18. The assays were carried out according to a 22 central composite design (CCD) with different concentrations of NaNO3 (1.25, 1.88 and 2.50 g L-1) and NaCl (1.00, 15.0 and 30.0 g L-1). The assays were carried out in 2 L vertical tubular photobioreactors at 30°C, 12 h light/dark and an injection of 12.0% v/v of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CO2; Fixation; Growth kinetics; Microalgae; Nutrients.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100410
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Carotenoids from Rhodomonas salina Induce Apoptosis and Sensitize A2058 Melanoma Cells to Chemotherapy ArchiMer
De Oliveira-júnior, Raimundo Gonçalves; Nicolau, Elodie; Bonnet, Antoine; Prunier, Grégoire; Beaugeard, Laureen; Joguet, Nicolas; Thiéry, Valérie; Picot, Laurent.
Melanoma is an aggressive tumor with invasive and metastatic potential, frequently exhibiting multidrug resistance mechanisms. In our continuous search for antimelanoma molecules, we have identified some effective marine compounds capable of not only inducing cell death, but also of sensitizing chemoresistant tumor cells to clinically used anticancer drugs. In this report, the cryptophyte Rhodomonas salina (Wislouch) D.R.A.Hill & R.Wetherbee, Pyrenomonadaceae, was chemically investigated in order to identify pigments efficiently inhibiting melanoma cells proliferation. All pharmacological tests were performed on A2058 cells expressing the oncogenic BRAF V600E mutation and resistant to dacarbazine treatment. Flash chromatography of R. salina ethanol...
Tipo: Text Palavras-chave: Alloxanthin; Chemosensitivity; Crocoxanthin; Cutaneous melanoma; Drug resistance; Microalgae.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00630/74194/73918.pdf
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Cassava processing wastewater as a platform for third generation biodiesel production Scientia Agricola
Neves,Cristina; Maroneze,Mariana Manzoni; Santos,Aline Meireles dos; Francisco,Erika Cristina; Wagner,Roger; Zepka,Leila Queiroz; Jacob-Lopes,Eduardo.
ABSTRACT This study aimed to evaluate third generation biodiesel production by microalgae Phormidium autumnale using cassava processing wastewater as a platform. Experiments were performed in a heterotrophic bubble column bioreactor. The study focused on the evaluation of the bioreactor (batch and fed-batch) of different operational modes and the analysis of biofuel quality. Results indicate that fed-batch cultivations improved system performance, elevating biomass and oil productions to 12.0 g L−1 and 1.19 g L−1, respectively. The composition of this oil is predominantly saturated (60 %) and monounsaturated (39 %), resulting in a biodiesel that complys with U.S., European and Brazilian standards. The technological route developed indicates potential for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microalgae; Cyanobacteria; Agroindustrial wastewater; Biofuel.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000500412
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Characterization of phototrophic microorganisms and description of new cyanobacteria isolated from the saline-alkaline crater-lake Dziani Dzaha (Mayotte, Indian Ocean) ArchiMer
Cellamare, M.; Duval, C.; Drelin, Y.; Djediat, C.; Touibi, N.; Agogue, H.; Leboulanger, C.; Ader, M.; Bernard, C.
The saline-alkaline crater-lake Dziani Dzaha (Mayotte, Indian Ocean) is dominated by the bloom-forming cyanobacterium Arthrospira. However, the rest of the phototrophic community remains underexplored because of their minute dimension or lower biomass. To characterize the phototrophic microorganisms living in this ecosystem considered as a modern analog of Precambrian environments, several strains were isolated from the water column and stromatolites, and analyzed using the polyphasic approach. Based on morphological, ultrastructural and molecular (16S rRNA gene, 18S rRNA gene, 16S-23S ITS region, cpcBA-IGS locus) methods, seven filamentous cyanobacteria and the prasinophyte Picocystis salinarum were identified. Two new genera and four new cyanobacteria...
Tipo: Text Palavras-chave: Cyanobacteria; Microalgae; Crater-lake; Saline-alkaline lake; Polyphasic approach; Arthrospira.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00443/55469/56991.pdf
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Combined Effects of Nitrogen Concentration and Seasonal Changes on the Production of Lipids in Nannochloropsis oculata ArchiMer
Olofsson, Martin; Lamela, Teresa; Nilsson, Emmelie; Berge, Jean-pascal; Del Pino, Victoria; Uronen, Pauliina; Legrand, Catherine.
Instead of sole nutrient starvation to boost algal lipid production, we addressed nutrient limitation at two different seasons (autumn and spring) during outdoor cultivation in flat panel photobioreactors. Lipid accumulation, biomass and lipid productivity and changes in fatty acid composition of Nannochloropsis oculata were investigated under nitrogen (N) limitation (nitrate:phosphate N:P 5, N:P 2.5 molar ratio). N. oculata was able to maintain a high biomass productivity under N-limitation compared to N-sufficiency (N:P 20) at both seasons, which in spring resulted in nearly double lipid productivity under N-limited conditions (0.21 g L−1 day−1) compared to N-sufficiency (0.11 g L−1 day−1). Saturated and monounsaturated fatty acids increased from 76% to...
Tipo: Text Palavras-chave: Microalgae; Outdoor; Nannochloropsis; Large-scale; Lipids; Fatty acids; Nitrogen manipulation; Seasonal changes; Biofuels; High value products.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00184/29564/27902.pdf
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Community structure of microfitobenthos from the central coastal zone of Venezuela Boletín de Investigaciones
Pereira,Carlos; De Roa,Evelyn Zoppi; Montiel,Edie; Hernández,Vanessa; Castillo,Anyoelis.
ABSTRACT Microphytobenthos is a community that groups benthic photosynthetic microorganisms. In Venezuela and the other countries in the Caribbean Sea, this group has been scarcely studied, so its diversity is unknown. This study aims to describe the spatial and temporal variations of its composition and abundance in the central coastal zone of Venezuela. Samplings were conducted monthly from June 2014 to March 2015, in random stations at depths less than 20 m. Richness and abundance data were processed through multivariate hypothesis tests (Anosim - SIMPER - nMDS). A total of 327 species were identified, with a dominance of diatoms and dinoflagellates, without significant spatial and temporal variations. In terms of abundance, the most representative...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Community structure; Marine ecology; Caribbean Sea; Microalgae.
Ano: 2022 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612022000200029
Registros recuperados: 122
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